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Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

DOI:
10.3791/58231-v

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08:15 min

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September 28, 2018

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, , , , , , , ,
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Chapters

  • 00:04Title
  • 00:46Seed Train Expansion and Automated Microbioreactor System Preparation
  • 02:55Automated Microbioreactor Software Initiation
  • 05:15System Shutdown
  • 05:58Cell Culture Harvest and IgG Titer Measurement
  • 07:04Results: Representative High-Throughput Automated Microbioreactor Analyses
  • 07:38Conclusion

Summary

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自动翻译

A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.

Tags

Keywords: High-throughputAutomated Microbioreactor SystemIgG1 ProductionCHO CellsCell Line CharacterizationProcess OptimizationShake FlasksAutomationSmall-scale StudiesCell CultureSubculturingCell CounterMicrobioreactor SoftwareTemperature ControlDissolved OxygenPH Monitoring

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