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Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

10,575 Views

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08:15 min

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September 28, 2018

DOI:

10.3791/58231-v

DOI:
10.3791/58231-v

•

08:15 min
September 28, 2018

•

8 Views
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1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Summary

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A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.

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Cite this Article

Velugula-Yellela, S. R., Kohnhorst, C., Powers, D. N., Trunfio, N., Faustino, A., Angart, P., Berilla, E., Faison, T., Agarabi, C. Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells. J. Vis. Exp. (139), e58231, doi:10.3791/58231 (2018).

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