Dergi
/
Gelişim Biyolojisi
/
Neurogenesis Using P19 Embryonal Carcinoma Cells
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Neurogenesis Using P19 Embryonal Carcinoma Cells

Neurogenesis Using P19 Embryonal Carcinoma Cells

DOI:
10.3791/58225-v

•

07:46 min

•

April 27, 2019

•

, , , , , , ,
1Department of Experimental Embryology, Institute of Genetics and Animal Breeding,Polish Academy of Sciences, 2Department of Regenerative Medicine,Maria Skłodowska-Curie Institute – Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding,Polish Academy of Sciences

Bölümler

  • 00:04Başlık
  • 00:27Culture Maintenance and Sub-culturing Cells
  • 02:13Trypsin Digestion
  • 03:29Aggregate Generation
  • 04:34Aggregate Dissociation
  • 05:47Plating Cells
  • 06:31Results: Neuronal Differentiation of P19 Cell Line
  • 07:23Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The P19 mouse embryonic carcinoma cell line (P19 cell line) is widely used for studying the molecular mechanism of neurogenesis with great simplification compared to in vivo analysis. Here, we present a protocol for retinoic acid-induced neurogenesis in the P19 cell line.

Etiketler

P19 Embryonal Carcinoma CellsNeurogenesisNeuron DifferentiationCell CultureTrypsin DigestionMaintenance MediumDifferentiation Medium

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Single-cell Photoconversion in Living Intact Zebrafish

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Read Article