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单分子力谱中蛋白质共价固定化的研究
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
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单分子力谱中蛋白质共价固定化的研究

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11:13 min

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August 20, 2018

DOI:

10.3791/58167-v

DOI:
10.3791/58167-v

•

11:13 min
August 20, 2018

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1Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, 3Center for Nano Science,Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

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本协议描述了 heterobifunctional 硅烷偶联剂在硅氧化物表面上的共价固定化, 其作用是以原子力显微镜为基础的单分子力光谱学为例。RrgA (pilus-1 尖黏附)与纤连蛋白的相互作用。

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Becke, T. D., Ness, S., Sudhop, S., Gaub, H. E., Hilleringmann, M., Schilling, A. F., Clausen-Schaumann, H. Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy. J. Vis. Exp. (138), e58167, doi:10.3791/58167 (2018).

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