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Detection of Viruses from Bioaerosols Using Anion Exchange Resin
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JoVE Journal Çevre
Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

DOI:
10.3791/58111-v

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06:10 min

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August 22, 2018

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, , , , , , , , , ,
1High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences,Colorado State University, 2National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service,United States Department of Agriculture, 3Western Sydney University, 4Leprino Foods, Inc, 5Department of Food Science and Agricultural Chemistry,McGill University, 6Makine Mühendisliği Bölümü,Colorado State University, 7Department of Animal Science,University of Wyoming

Bölümler

  • 00:04Başlık
  • 00:30Bioaerosol Chamber Setup and Model Bioaerosolization Experiments
  • 02:58Nucleic Acid Extraction and qRT-PCR Detection
  • 04:10Results: Anion Exchange Resin Improves qRT-PCR-based, Negatively-charged Virus Bioaerosol Detection
  • 04:46Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

An anion exchange resin-based method, adapted to liquid impingement-based bioaerosol sampling of viruses is demonstrated. When coupled with downstream molecular detection, the method allows for facile and sensitive detection of viruses from bioaerosols.

Etiketler

Virus DetectionBioaerosolsAnion Exchange ResinMolecular DetectionPublic HealthVeterinary HealthViral ParticlesCollision NebulizerLiquid ImpingersHEPA Filtered AirAir ContaminantsSamplingCalibration

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