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וזמינותו אופטי למיחזור שלפוחית סינפטית בנוירונים בתרבית Overexpressing חלבונים Presynaptic

JoVE Journal
Nörobilim

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JoVE Journal Nörobilim

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

Please note that all translations are automatically generated. Click here for the English version.

וזמינותו אופטי למיחזור שלפוחית סינפטית בנוירונים בתרבית Overexpressing חלבונים Presynaptic

7,264 Views

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09:33 min

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June 26, 2018

DOI:

10.3791/58043-v

DOI:

10.3791/58043-v

•

09:33 min

June 26, 2018

•

3 Views

Donatus Riemann, Andoniya Petkova, Thomas Dresbach, Rebecca Wallrafen

¹Institute for Anatomy and Embryology,University Medical Centre Göttingen

Etiketler

Optical Assay Synaptic Vesicle Recycling Cultured Neurons Presynaptic Proteins Antibody Specificity Primary Hippocampal Cells Transfection Calcium Chloride Endotoxin-free DNA Transfection Buffer Reduced-serum Medium Cell-culture Medium Incubation Washing Active Synapses

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