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An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

JoVE Revista
Neurociencias

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JoVE Revista Neurociencias

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

7,264 Views

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09:33 min

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June 26, 2018

DOI:

10.3791/58043-v

DOI:

10.3791/58043-v

•

09:33 min

June 26, 2018

•

3 Views

Donatus Riemann, Andoniya Petkova, Thomas Dresbach, Rebecca Wallrafen

¹Institute for Anatomy and Embryology,University Medical Centre Göttingen

Tags

Optical Assay Synaptic Vesicle Recycling Cultured Neurons Presynaptic Proteins Antibody Specificity Primary Hippocampal Cells Transfection Calcium Chloride Endotoxin-free DNA Transfection Buffer Reduced-serum Medium Cell-culture Medium Incubation Washing Active Synapses

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