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Overexpressing 突触前蛋白培养神经元突触囊泡再生的光学检测
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JoVE Journal Nörobilim
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
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Overexpressing 突触前蛋白培养神经元突触囊泡再生的光学检测

7,264 Views

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09:33 min

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June 26, 2018

DOI:

10.3791/58043-v

DOI:
10.3791/58043-v

•

09:33 min
June 26, 2018

•

3 Views
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1Institute for Anatomy and Embryology,University Medical Centre Göttingen

Etiketler

Optical AssaySynaptic Vesicle RecyclingCultured NeuronsPresynaptic ProteinsAntibody SpecificityPrimary Hippocampal CellsTransfectionCalcium ChlorideEndotoxin-free DNATransfection BufferReduced-serum MediumCell-culture MediumIncubationWashingActive Synapses

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Riemann, D., Petkova, A., Dresbach, T., Wallrafen, R. An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins. J. Vis. Exp. (136), e58043, doi:10.3791/58043 (2018).

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