We present a protocol for measuring oxygen consumption and extracellular acidification in Drosophila melanogaster larval and adult brains. A metabolic analyzer is utilized with an adapted and optimized protocol. Micro-tissue restraints are a critical component of this protocol and were designed and created specifically for their use in this analysis.
Neville, K. E., Bosse, T. L., Klekos, M., Mills, J. F., Tipping, M. Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains. J. Vis. Exp. (138), e58007, doi:10.3791/58007 (2018).