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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

56,616 Views

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09:12 min

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October 17, 2018

DOI:

10.3791/57941-v

DOI:
10.3791/57941-v

•

09:12 min
October 17, 2018

•

37 Views
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1Department of Surgery, Vascular Biology Research Centre,Westmead Hospital, 2Westmead Clinical School, Department of Surgery,The University of Sydney, 3Westmead Research Hub,Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research,Westmead Hospital

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Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of the expression of M1 (inflammatory) and M2 markers (anti-inflammatory).

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Monocyte SubsetsFlow CytometryWhole Blood AnalysisCD14CD16HLA-DRM1 And M2 MarkersGatingRed Blood Cell LysisWhite Blood Cell FixationSample PreparationFlow Cytometry SetupQuality Control

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Marimuthu, R., Francis, H., Dervish, S., Li, S. C., Medbury, H., Williams, H. Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis. J. Vis. Exp. (140), e57941, doi:10.3791/57941 (2018).

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