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Métabolisme de cartographie : Surveiller l’activité de la Lactate déshydrogénase directement dans le tissu
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JoVE Journal Biyokimya
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
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Métabolisme de cartographie : Surveiller l’activité de la Lactate déshydrogénase directement dans le tissu

11,089 Views

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06:18 min

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June 21, 2018

DOI:

10.3791/57760-v

DOI:
10.3791/57760-v

•

06:18 min
June 21, 2018

•

10 Views
, , , , , , , ,
1Department of Molecular, Cell and Developmental Biology,UCLA, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,UCLA

Özet

Automatically generated

Les auteurs décrivent un protocole pour cartographier la distribution spatiale de l’activité enzymatique d’une enzyme qui génèrent nicotinatmide adénine dinucléotide phosphate (NAD(P)H) + H + directement dans les échantillons de tissus.

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Cite this Article

Jelinek, D., Flores, A., Uebelhoer, M., Pasque, V., Plath, K., Iruela-Arispe, M. L., Christofk, H. R., Lowry, W. E., Coller, H. A. Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue. J. Vis. Exp. (136), e57760, doi:10.3791/57760 (2018).

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