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2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
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August 06, 2018
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Protein-protein and protein-metabolite interactions are crucial for all cellular functions. Herein, we describe a protocol that allows parallel analysis of these interactions with a protein of choice. Our protocol was optimized for plant cell cultures and combines affinity purification with mass spectrometry-based protein and metabolite detection.
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Affinity PurificationProtein-protein ComplexesProtein-metabolite ComplexesParallel AnalysisBasic ResearchMedicalPharmaceuticalBiotechnologicalSmall Molecular LigandsIn Vivo ConditionsInteracting MoleculesPSBL Arabidopsis ThalianaCell CultureMSMO MediumLogarithmic Growth PhaseMixer MillLysis BufferIgG Sepharose Beads
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Luzarowski, M., Wojciechowska, I., Skirycz, A. 2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes. J. Vis. Exp. (138), e57720, doi:10.3791/57720 (2018).
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