Dergi
/
Biyoloji
/
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

DOI:
10.3791/57643-v

•

12:52 min

•

May 12, 2018

•

,
1Division of Molecular Cell Biology, Zoological Institute,Technische Universität Braunschweig, 2Department of Cell Biology,Helmholtz Centre for Infection Research

Bölümler

  • 00:05Başlık
  • 00:50Treatment of Cells, Transfection, and Seeding onto Coverslips
  • 02:54Assembly of Microscopy Imaging Chamber
  • 03:43Microinjection Procedure
  • 05:02FRAP Procedure
  • 05:32Photoactivation Procedure
  • 06:26Data Analysis
  • 09:42Results: Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
  • 11:27Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe how micro- and photomanipulation techniques such as FRAP and photoactivation enable the determination of motility parameters and the spatiotemporal dynamics of proteins within migrating cells. Experimental readouts include subcellular dynamics and turnover of motility regulators or of the underlying actin cytoskeleton.

Etiketler

MicromanipulationCytoskeletal RegulatorsFRAPPhotoactivationB16-F1 CellsNIH 3T3 FibroblastsLamininFibronectinTransfectionMicroscopy

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Simple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection

Identification of Novel Regulators of Plant Transpiration by Large-Scale Thermal Imaging Screening in Helianthus Annuus

Read Article