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单细胞基因组测序的超高通量微流控平台
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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单细胞基因组测序的超高通量微流控平台

17,100 Views

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10:00 min

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May 23, 2018

DOI:

10.3791/57598-v

DOI:
10.3791/57598-v

•

10:00 min
May 23, 2018

•

25 Views
, , ,
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, San Francisco, 2UC Berkeley-UCSF Graduate Program in Bioengineering,University of California, San Francisco, 3Chan Zuckerberg Biohub

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单细胞测序揭示了生物系统中的基因型异质性, 但目前的技术缺乏对群落组成和功能的深入分析所必需的吞吐量。在这里, 我们描述了一个微流控流程的序列 > 5万单细胞基因组从不同的细胞群。

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Demaree, B., Weisgerber, D., Lan, F., Abate, A. R. An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing. J. Vis. Exp. (135), e57598, doi:10.3791/57598 (2018).

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