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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Bioingegneria
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JoVE Journal Bioingegneria
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

17,114 Views

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10:00 min

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May 23, 2018

DOI:

10.3791/57598-v

DOI:
10.3791/57598-v

•

10:00 min
May 23, 2018

•

25 Views
, , ,
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, San Francisco, 2UC Berkeley-UCSF Graduate Program in Bioengineering,University of California, San Francisco, 3Chan Zuckerberg Biohub

Summary

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Single-cell sequencing reveals genotypic heterogeneity in biological systems, but current technologies lack the throughput necessary for the deep profiling of community composition and function. Here, we describe a microfluidic workflow for sequencing >50,000 single-cell genomes from diverse cell populations.

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Demaree, B., Weisgerber, D., Lan, F., Abate, A. R. An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing. J. Vis. Exp. (135), e57598, doi:10.3791/57598 (2018).

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