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Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
JoVE Journal
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Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

DOI:
10.3791/57463-v

•

06:52 min

•

May 21, 2018

•

,
1Department of Laboratory Medicine,Washington Üniversitesi

'Bölümler'

  • 00:04'Başlık'
  • 00:45NOD-like RecptorP3 (NLRP3) Inflammasome Activation, Caspase-1 Activity Probe (FAM-YVAD-FMK)-labeling, and Antibody Staining
  • 04:07Lactate Dehydrogenase (LDH) Release Assay
  • 05:23Results: Representative Inflammasome Activation and Pyroptotic Cell Death Detection
  • 06:21Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

We describe the detection of NLRP3 inflammasome activation on cellular basis using fluorescence microscopy and staining for active caspase-1 and the adaptor, ASC. A lactate dehydrogenase release assay is presented to detect pyroptotic lysis on a population basis. These techniques can be adapted to study many aspects of inflammasome biology.

'Etiketler'

Inflammasome ActivationPyroptosisMacrophagesFluorescence MicroscopyLDH Release AssayCaspase-1NigericinFAM-YVAD-FMKParaformaldehydeConfocal Microscopy

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