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小鼠和人源性胃上皮单层培养对再生的研究
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JoVE Journal İmmünoloji ve Enfeksiyon
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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小鼠和人源性胃上皮单层培养对再生的研究

10,259 Views

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11:48 min

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May 07, 2018

DOI:

10.3791/57435-v

DOI:
10.3791/57435-v

•

11:48 min
May 07, 2018

•

12 Views
, , , ,
1Department of Pharmacology and Systems Physiology,University of Cincinnati, 2Department of Cell & Developmental Biology,University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Özet

Automatically generated

在这里, 我们描述了建立和培养人和老鼠衍生的3维 (3D) 胃 organoids 的协议, 以及将 3D organoids 转移到2维单层的方法。本文还介绍了用胃上皮单层作为再生研究的一种新的擦伤创面检测方法。

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Teal, E., Steele, N. G., Chakrabarti, J., Holokai, L., Zavros, Y. Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration. J. Vis. Exp. (135), e57435, doi:10.3791/57435 (2018).

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