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基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响
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A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells
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基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响

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11:51 min

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July 05, 2018

DOI:

10.3791/57361-v

DOI:
10.3791/57361-v

•

11:51 min
July 05, 2018

•

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, , ,
1Department of Biochemistry,Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering,University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute,Stanford University School of Medicine

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我们开发了一种多井格式的聚丙烯酰胺检测方法, 以探讨细胞外基质硬度对黏附细胞细菌感染的影响。该检测与流式细胞术、染色和牵引力显微镜相兼容, 可以定量测量细胞、胞外基质和致病细菌之间的生物力学相互作用。

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PolyacrylamideExtracellular MatrixStiffnessBacterial InfectionAdherent CellsHost-pathogen BiomechanicsHydrogelsAcrylamideBis-acrylamideTEMEDAPSFluorescent Microbeads

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Bastounis, E. E., Ortega, F. E., Serrano, R., Theriot, J. A. A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells. J. Vis. Exp. (137), e57361, doi:10.3791/57361 (2018).

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