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A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

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11:51 min

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July 05, 2018

DOI:

10.3791/57361-v

DOI:
10.3791/57361-v

•

11:51 min
July 05, 2018

•

4 Views
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1Department of Biochemistry,Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering,University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute,Stanford University School of Medicine

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We have developed a multi-well format polyacrylamide-based assay for probing the effect of extracellular matrix stiffness on bacterial infection of adherent cells. This assay is compatible with flow cytometry, immunostaining, and traction force microscopy, allowing for quantitative measurements of the biomechanical interactions between cells, their extracellular matrix, and pathogenic bacteria.

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PolyacrylamideExtracellular MatrixStiffnessBacterial InfectionAdherent CellsHost-pathogen BiomechanicsHydrogelsAcrylamideBis-acrylamideTEMEDAPSFluorescent Microbeads

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Bastounis, E. E., Ortega, F. E., Serrano, R., Theriot, J. A. A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells. J. Vis. Exp. (137), e57361, doi:10.3791/57361 (2018).

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