In this protocol, we describe the selection and preparation of appropriate cells for micromanipulation and the use of a piezoelectric micromanipulator to reposition chromosomes within those cells.
Lin, N. K., Nance, R., Szybist, J., Cheville, A., Paliulis, L. V. Micromanipulation of Chromosomes in Insect Spermatocytes. J. Vis. Exp. (140), e57359, doi:10.3791/57359 (2018).