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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
JoVE Journal
Genetik
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JoVE Journal Genetik
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

DOI:
10.3791/57320-v

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10:16 min

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June 28, 2018

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, , , ,
1Nuclear Dynamics Programme, The Babraham Institute,Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol,Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute,Babraham Research Campus, 5Department of Biological Science,Florida State University

Bölümler

  • 00:04Başlık
  • 00:52Formaldehyde Fixation
  • 02:14HindIII Digestion and Biotinylation of Restriction Fragment Overhangs
  • 04:09Hybrid In-solution Capture
  • 06:53Isolation of Promoter Fragment-containing Ligation Products
  • 08:55Results: IL8 PCHi-C Profile in Human Hematopoietic Cells
  • 09:34Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

DNA regulatory elements, such as enhancers, control gene expression by physically contacting target gene promoters, often through long-range chromosomal interactions spanning large genomic distances. Promoter Capture Hi-C (PCHi-C) identifies significant interactions between promoters and distal regions, enabling the assignment of potential regulatory sequences to their target genes.

Etiketler

Promoter Capture Hi-CGenome-wide ProfilingPromoter InteractionsGene Regulatory ElementsTarget GenesGenome StructureGene Expression ControlNoncoding Disease VariantsRegulatory Control SequencesCell LysisRestriction BufferCell Nuclei

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