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Miniaturized Sample Preparation for Transmission Electron Microscopy
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Miniaturized Sample Preparation for Transmission Electron Microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

18,929 Views

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09:04 min

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July 27, 2018

DOI:

10.3791/57310-v

DOI:
10.3791/57310-v

•

09:04 min
July 27, 2018

•

10 Views
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1Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum,Basel Üniversitesi, 2Swiss Nanoscience Institute,Basel Üniversitesi, 3BioEM lab, Biozentrum,Basel Üniversitesi

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An instrument and methods for the preparation of nanoliter-sized sample volumes for transmission electron microscopy is presented. No paper-blotting steps are required, thus avoiding the detrimental consequences this can have for proteins, significantly reducing sample loss and enabling the analysis of single cell lysate for visual proteomics.

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Schmidli, C., Rima, L., Arnold, S. A., Stohler, T., Syntychaki, A., Bieri, A., Albiez, S., Goldie, K. N., Chami, M., Stahlberg, H., Braun, T. Miniaturized Sample Preparation for Transmission Electron Microscopy. J. Vis. Exp. (137), e57310, doi:10.3791/57310 (2018).

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