Dergi
/
Biyokimya
/
洗剤に敏感な膜タンパク質相互作用の検出
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Detection of Detergent-sensitive Interactions Between Membrane Proteins
Please note that all translations are automatically generated. Click here for the English version.

洗剤に敏感な膜タンパク質相互作用の検出

5,826 Views

•

10:09 min

•

March 07, 2018

DOI:

10.3791/57179-v

DOI:
10.3791/57179-v

•

10:09 min
March 07, 2018

•

2 Views
, ,
1Department of Biochemistry,Boston University School of Medicine

Özet

Automatically generated

洗剤に敏感な例として並べ替え受容体、ソーティリン、GLUT4 グルコース輸送体タンパク質の最初の内腔のループに結合を用いた膜タンパク質相互作用の検出のためのプロトコルについて述べる。

Explore More Videos

Membrane ProteinsDetergent-sensitive InteractionsHistidine-taggedMyc-taggedCo-immunoprecipitationCell Lysate3T3-L1 Pre-adipocytesTransfectionProtein InteractionsBiyokimya

Read Article

Cite this Article

Zaarur, N., Pan, X., Kandror, K. V. Detection of Detergent-sensitive Interactions Between Membrane Proteins. J. Vis. Exp. (133), e57179, doi:10.3791/57179 (2018).

Download .ris file

Copy

Share Video

.

Copy