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Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
Bölümler
- 00:04Başlık
- 00:56Parameters, Solution Setup, and Micro-dosage Systems Adjustment
- 02:42Seeing a Thaumatin Drop in the Experimental Chamber and in Situ Measurements
- 04:48Sample Evaporation, Precipitant Addition, and Tracking the Evolution of the Crystallization Droplet Over Time
- 06:12Recovery of Crystallization Droplet
- 07:09Results: Formation of Protein Microcrystals from T. daniellii Thaumatin
- 07:57Conclusion
Here we present a protocol for controlled production of protein microcrystals. The process uses an automated device allowing controlled manipulation of several crystallization parameters. The protein crystallization is carried-out by controlled and automated addition of crystallization solutions while monitoring and investigating the radius distribution of particles in the crystallization droplet.
