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Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels
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JoVE Journal Nörobilim
Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels

Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels

DOI:
10.3791/57052-v

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09:55 min

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February 14, 2018

•

,
1Biyoloji Bölümü,University of Maryland

Bölümler

  • 00:04Başlık
  • 00:42Preparation
  • 03:17Obtaining a Whole-cell Recording Configuration
  • 05:36Testing for Synaptic Connectivity
  • 07:17Results: Using Tetrotoxin Engineered Resistance for Probing Synapses (TERPS)
  • 08:58Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This article features a method to test the monosynaptic connections between neurons by employing tetrodotoxin and the tetrodotoxin-resistant sodium channel, NaChBac.

Etiketler

Monosynaptic ConnectionsDrosophilaTetrodotoxin Resistant Sodium ChannelsElektrofizyolojiDissectionPatch-clamp RecordingsRecording SalineCsChrimsonAll-trans-RetinalAntennal LobesGlial Sheathing

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