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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

23,393 Views

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09:35 min

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February 26, 2018

DOI:

10.3791/57038-v

DOI:
10.3791/57038-v

•

09:35 min
February 26, 2018

•

59 Views
, , ,
1Institute of Neurology (Edinger Institute),Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology,Goethe University Hospital

Özet

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Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.

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Devraj, K., Guérit, S., Macas, J., Reiss, Y. An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers. J. Vis. Exp. (132), e57038, doi:10.3791/57038 (2018).

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