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蛍光寿命イメージングによる細胞内のスネアを可視化
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
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蛍光寿命イメージングによる細胞内のスネアを可視化

9,197 Views

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08:55 min

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December 29, 2017

DOI:

10.3791/56745-v

DOI:
10.3791/56745-v

•

08:55 min
December 29, 2017

•

7 Views
, , ,
1Department of Tumor Immunology,Radboud University Medical Center

Özet

Automatically generated

このプロトコルでは、蛍光共鳴エネルギー移動し、蛍光寿命イメージングに基づく SNARE タンパク質の複合体形成の定量的可視化を可能にする新しいメソッドについて説明します。

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Cite this Article

Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).

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