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Visualizing intracellulare rullante traffico da fluorescenza Lifetime Imaging microscopia
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
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Visualizing intracellulare rullante traffico da fluorescenza Lifetime Imaging microscopia

9,197 Views

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08:55 min

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December 29, 2017

DOI:

10.3791/56745-v

DOI:
10.3791/56745-v

•

08:55 min
December 29, 2017

•

7 Views
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1Department of Tumor Immunology,Radboud University Medical Center

Özet

Automatically generated

Questo protocollo descrive un nuovo metodo che consente la visualizzazione quantitativa della formazione di complessi di proteine SNARE, basato sul trasferimento di energia per risonanza e la durata di fluorescenza imaging microscopia.

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Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).

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