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荧光寿命成像显微镜观察细胞内圈套的贩运
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
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荧光寿命成像显微镜观察细胞内圈套的贩运

9,197 Views

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08:55 min

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December 29, 2017

DOI:

10.3791/56745-v

DOI:
10.3791/56745-v

•

08:55 min
December 29, 2017

•

7 Views
, , ,
1Department of Tumor Immunology,Radboud University Medical Center

Özet

Automatically generated

该协议描述了一种新的方法, 允许定量可视化的复杂形成的圈套蛋白, 基于 rster 共振能量转移, 和荧光寿命成像显微镜。

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Cite this Article

Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).

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