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06:43 min
February 05, 2018
DOI:
10.3791/56647-v
该协议提供了一个体外活体成像吞噬试验来测量星形胶质细胞的吞噬能力。纯化大鼠星形胶质细胞和小胶质和 pH 指标-共轭突。该方法可检测实时吞没和降解动力学, 为确定影响星形胶质细胞吞噬能力的因素提供了一个合适的筛选平台。
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Byun, Y. G., Chung, W. A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes. J. Vis. Exp. (132), e56647, doi:10.3791/56647 (2018).
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