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A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes
 

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Article DOI: 10.3791/56647-v • 06:43 min • February 5th, 2018
February 5th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.

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In Vitro Live-imaging Assay Astrocyte-mediated Phagocytosis PH Indicator-conjugated Synaptosomes Glial Cells Neuroscience Real Time Kinetics In Vitro Cultured Cells Factors And Compounds Centrifugation Sodium Carbonate PH Indicator Aluminum Foil Covers Twist Shaker Agitation Incubation Dulbecco's PBS (DPBS)
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