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Détection rapide des phénotypes de développement neurologique dans des cellules précurseurs neurales humaines (PNJ)
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JoVE Journal Gelişim Biyolojisi
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
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Détection rapide des phénotypes de développement neurologique dans des cellules précurseurs neurales humaines (PNJ)

9,804 Views

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10:47 min

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March 02, 2018

DOI:

10.3791/56628-v

DOI:
10.3791/56628-v

•

10:47 min
March 02, 2018

•

8 Views
, , , , , , , , ,
1Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services,Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology,Rutgers Robert Wood Johnson Medical School, 5Genetik Bölümü,Rutgers University

Özet

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Sur le développement neurologique des processus tels que la prolifération, la migration et la croissance des neurites sont souvent perturbées dans les maladies neuropsychiatriques. Ainsi, nous présentons des protocoles afin de rapidement et de façon reproductible évaluer ces processus neurologique dans humain iPSC dérivés PNJ. Ces protocoles permettent également l’évaluation des effets des facteurs de croissance et de la thérapeutique sur le développement de NPC.

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Williams, M., Prem, S., Zhou, X., Matteson, P., Yeung, P. L., Lu, C., Pang, Z., Brzustowicz, L., Millonig, J. H., Dicicco-Bloom, E. Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs). J. Vis. Exp. (133), e56628, doi:10.3791/56628 (2018).

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