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Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry
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JoVE Journal Nörobilim
Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

11,215 Views

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12:34 min

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December 06, 2017

DOI:

10.3791/56538-v

DOI:
10.3791/56538-v

•

12:34 min
December 06, 2017

•

6 Views
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1Department of Medicine I and Clinical Chemistry,University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich,Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD)

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Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.

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Flow CytometryImmune CellsSciatic NerveDorsal Root GanglionNeuropathyNeuroinflammationMouseDissectionCell IsolationDigestion

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Cite this Article

Hidmark, A. S., Nawroth, P. P., Fleming, T. Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry. J. Vis. Exp. (130), e56538, doi:10.3791/56538 (2017).

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