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Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
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Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

7,710 Views

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07:12 min

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October 26, 2017

DOI:

10.3791/56527-v

DOI:
10.3791/56527-v

•

07:12 min
October 26, 2017

•

5 Views
,
1Department of Chemistry and Chemical Biology,Cornell University

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A protocol for the synthesis, purification, and characterization of a ruthenium-based inhibitor of mitochondrial calcium uptake is presented. A procedure to evaluate its efficacy in permeabilized mammalian cells is demonstrated.

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Cite this Article

Nathan, S. R., Wilson, J. J. Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor. J. Vis. Exp. (128), e56527, doi:10.3791/56527 (2017).

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