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化细胞穿透肽研究胞内蛋白-蛋白质相互作用
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
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化细胞穿透肽研究胞内蛋白-蛋白质相互作用

9,070 Views

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10:26 min

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December 20, 2017

DOI:

10.3791/56457-v

DOI:
10.3791/56457-v

•

10:26 min
December 20, 2017

•

10 Views
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1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular,Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences,Queen’s University Belfast

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Automatically generated

这是一项研究细胞内蛋白-蛋白质相互作用的协议, 基于生物素-素下拉系统, 具有新颖性的单元穿透序列结合。主要的优点是, 目标序列是用活细胞来培养的, 而不是细胞裂解, 因此相互作用将发生在细胞环境中。

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Jaraíz-Rodríguez, M., González-Sánchez, A., García-Vicente, L., Medina, J. M., Tabernero, A. Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions. J. Vis. Exp. (130), e56457, doi:10.3791/56457 (2017).

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