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Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

DOI:
10.3791/56441-v

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09:17 min

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April 12, 2018

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, , ,
1Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center,University of Zurich

Bölümler

  • 00:04Başlık
  • 00:56Flower and Silique Fixation and Dissection Under the Stereomicroscope
  • 02:05Chloral Hydrate-based Clearing and Combined Clearing-staining
  • 03:28Combined Alexander Staining and Herr’s 4½ Clearing of Anthers
  • 04:29Removing the Exine from Pollen Grains
  • 05:49Sodium Dodecyl Sulfate (SDS) Clearing
  • 07:04Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures
  • 08:41Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive structures.

Etiketler

ArabidopsisFlower OrgansSiliquesWhole-mount ClearingStainingDissectionFlower DevelopmentSexual Plant ReproductionMicroscopyClearing Solution

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