/
/
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
6,386 Views
•
07:35 min
•
August 03, 2017
DOI:
1Sackler Faculty of Medicine,Tel Aviv Üniversitesi, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine,Tel Aviv Üniversitesi, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine,Tel Aviv Üniversitesi, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine,Tel Aviv Üniversitesi
Özet
Automatically generated
A simple protocol for overexpression and purification of codon-optimized, human cis-prenyltransferase, under non-denaturing conditions, from Escherichia coli, is described, along with an enzymatic activity assay. This protocol can be generalized for production of other cis- prenyltransferase proteins in quantity and quality suitable for mechanistic studies.
Read Article
Cite this Article
Edri, I., Goldenberg, M., Lisnyansky, M., Strulovich, R., Newman, H., Loewenstein, A., Khananshvili, D., Giladi, M., Haitin, Y. Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli. J. Vis. Exp. (126), e56430, doi:10.3791/56430 (2017).
Copy