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Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
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Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
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October 30, 2017
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This protocol describes how to produce retinal pigment epithelial cells (RPE) from pluripotent stem cells. The method uses a combination of growth factors and small molecules to direct the differentiation of stem cells into immature RPE in fourteen days and mature, functional RPE after three months.
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Retinal Pigment Epithelial CellsHuman Embryonic Stem CellsInduced Pluripotent Stem CellsRetinal DifferentiationDirected DifferentiationStem Cell BiologyEye ResearchRPERetinal DevelopmentStem Cell Disease ModelGrowth FactorsSmall MoleculesExtracellular MatrixHydrogelEDTACell PassagingCell RemovalUndifferentiated Stem CellsEfficiencyEnrichment
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Foltz, L. P., Clegg, D. O. Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells. J. Vis. Exp. (128), e56274, doi:10.3791/56274 (2017).
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