The goal of the protocol is to prepare keratin hydrolysate from chicken feathers by alkaline-enzymatic hydrolysis and to test whether adding keratin hydrolysate into a cosmetics ointment base improves skin barrier function (heightening hydration and diminishing transepidermal water loss). Tests are conducted on men and woman volunteers.
Mokrejš, P., Huťťa, M., Pavlačková, J., Egner, P. Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics. J. Vis. Exp. (129), e56254, doi:10.3791/56254 (2017).