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Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
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Genetik
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JoVE Journal Genetik
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

10,850 Views

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10:43 min

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June 03, 2017

DOI:

10.3791/55836-v

DOI:
10.3791/55836-v

•

10:43 min
June 03, 2017

•

1 Views
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1Basic Sciences Division,Fred Hutchinson Cancer Research Center, 2Biyoloji Bölümü,Indiana Üniversitesi

Özet

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We describe chromatin endogenous cleavage coupled with high-throughput sequencing (ChEC-seq), a chromatin immunoprecipitation (ChIP)-orthogonal method for mapping protein binding sites genome-wide with micrococcal nuclease (MNase) fusion proteins.

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ChEC-seqProtein-DNA InteractionsGenome-wide MappingSaccharomyces CerevisiaeTranscriptionChromatinDNA BindingRegulatory FactorsHigh ResolutionLow BackgroundFormaldehyde CrosslinkingChromatin SolubilizationAntibodiesMNaseCalcium AdditionStop SolutionSDS

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Cite this Article

Grünberg, S., Zentner, G. E. Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae. J. Vis. Exp. (124), e55836, doi:10.3791/55836 (2017).

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