Laboratory Techniques Used to Maintain and Differentiate Biotypes of <em>Vibrio cholerae</em> Clinical and Environmental Isolates

Kyle D. Brumfield; Bailey M. Carignan; Jordan N. Ray; Panagiota E. Jumpre; Mike S. Son

doi:10.3791/55760

/

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

DOI:

10.3791/55760-v

•

07:58 min

•

May 30, 2017

•

Kyle D. Brumfield*¹, Bailey M. Carignan*¹, Jordan N. Ray, Panagiota E. Jumpre, Mike S. Son

¹Department of Biological Sciences,Plymouth State University

Bölümler

00:05Başlık
01:28Preparation and Use of Frozen Stock Cultures
02:46Growth of Overnight Cultures in Liquid Medium
03:23Phenotypic Assays for Biotype Classification via Spotting
04:04Motility Assay
04:48Voges-Proskauer (VP) Assay
05:47Results: Reliable Distinction Between Biotypes
07:08Conclusion

Özet

Otomatik Çeviri

This manuscript describes proper Vibrio cholerae maintenance techniques in addition to a series of biochemical assays, collectively utilized for quick and reliable differentiation between clinical and environmental V. cholerae biotypes in a laboratory setting.

Etiketler

Vibrio Cholerae Clinical Isolates Environmental Isolates Biotyping Genotypic Characterization Phenotypic Characterization Biochemical Assays Metabolic Capabilities Enteric Bacteria Laboratory Techniques Culture Maintenance Cryogenic Storage Agar Plating Liquid Culture Inoculation

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