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分离的原发性上皮细胞从附睾的全细胞贴片钳记录
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JoVE Journal Gelişim Biyolojisi
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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分离的原发性上皮细胞从附睾的全细胞贴片钳记录

10,826 Views

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11:52 min

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August 03, 2017

DOI:

10.3791/55700-v

DOI:
10.3791/55700-v

•

11:52 min
August 03, 2017

•

2 Views
, , , ,
1School of Life Science and Technology,ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University

Özet

Automatically generated

我们提出了一种协议，结合细胞分离和全细胞膜片钳记录来测量主要解离的上皮细胞从大鼠cauda附睾的电性质。该方案允许研究原发性附睾上皮细胞的功能特性，以进一步阐明附睾的生理作用。

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Cite this Article

Zhang, B. L., Gao, D. Y., Zhang, X. X., Shi, S., Shum, W. Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis. J. Vis. Exp. (126), e55700, doi:10.3791/55700 (2017).

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