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Developmental Biology

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JoVE Journal Developmental Biology
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
 

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Article DOI: 10.3791/55700-v • 11:52 min • August 3rd, 2017
August 3rd, 2017 •
1,2,3, 1,2,3, 1,3, 4, 1
1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

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We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.

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Whole-cell Patch-clamp Recordings Isolated Primary Epithelial Cells Epididymis Electrical Properties Rat Reproductive Biology Sperm Maturation Paternal Traits Cauterize Physiological Function Bao Li Zhang Grad Student Disinfect Dissection Tools IMDM Aliquots Aseptic Conditions Collagenous Enzyme Digestion Solution Collagenase Type I Collagenase Type II RPMI Solution
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