Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
15,883 Views
•
10:13 min
•
June 09, 2017
DOI:
1The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery,Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences,Johns Hopkins University School of Medicine
Özet
Automatically generated
Guanosine triphosphate (GTP) binding is one of the earliest events in G-Protein-Coupled Receptor (GPCR) activation. This protocol describes how to pharmacologically characterize specific GPCR-ligand interactions by monitoring the binding of the radio-labeled GTP analog, [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPγS), in response to a ligand of interest.
Read Article
Cite this Article
Vasavda, C., Zaccor, N. W., Scherer, P. C., Sumner, C. J., Snyder, S. H. Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding. J. Vis. Exp. (124), e55561, doi:10.3791/55561 (2017).
Copy