Dergi
/
Biyokimya
/
Hoge resolutie single particle analyse van elektroncryo-microscopie beelden met behulp van SPHIRE
JoVE Journal
Biyokimya
This content is Free Access.
JoVE Journal Biyokimya
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Please note that all translations are automatically generated. Click here for the English version.

Hoge resolutie single particle analyse van elektroncryo-microscopie beelden met behulp van SPHIRE

49,814 Views

•

13:28 min

•

May 16, 2017

DOI:

10.3791/55448-v

DOI:
10.3791/55448-v

•

13:28 min
May 16, 2017

•

12 Views
, , , , , , , ,
1Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, 2Biyokimya ve Moleküler Biyoloji Anabilim Dalı,The University of Texas Medical School at Houston

Özet

Automatically generated

In dit artikel wordt een protocol voor het verwerken van cryo-EM-beelden gepresenteerd met behulp van de software suite SPHIRE. Het onderhavige protocol kan toegepast worden op bijna alle enkel-deeltjes-EM-projecten die zich richten op bijna atomaire resolutie.

Explore More Videos

Single Particle AnalysisElectron Cryo-microscopySPHIREMacromolecular ComplexesHigh-resolution Cryo-EMParticle Box SizeCTF EstimationMotion CorrectionDose-weightedDose-unweightedParticle Picking

Read Article

Cite this Article

Moriya, T., Saur, M., Stabrin, M., Merino, F., Voicu, H., Huang, Z., Penczek, P. A., Raunser, S., Gatsogiannis, C. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J. Vis. Exp. (123), e55448, doi:10.3791/55448 (2017).

Download .ris file

Copy

Share Video

.

Copy