Dergi
/
Biyokimya
/
SPHIREを用いた電子冷凍顕微鏡画像からの高分解能単一粒子解析
JoVE Journal
Biyokimya
This content is Free Access.
JoVE Journal Biyokimya
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Please note that all translations are automatically generated. Click here for the English version.

SPHIREを用いた電子冷凍顕微鏡画像からの高分解能単一粒子解析

49,814 Views

•

13:28 min

•

May 16, 2017

DOI:

10.3791/55448-v

DOI:
10.3791/55448-v

•

13:28 min
May 16, 2017

•

12 Views
, , , , , , , ,
1Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, 2Biyokimya ve Moleküler Biyoloji Anabilim Dalı,The University of Texas Medical School at Houston

Özet

Automatically generated

本稿では、ソフトウェアスイートSPHIREを使用して低温EM画像を処理するためのプロトコルを紹介します。現在のプロトコルは、ほぼ原子分解能を目標とするほぼすべての単一粒子EMプロジェクトに適用することができます。

Explore More Videos

Single Particle AnalysisElectron Cryo-microscopySPHIREMacromolecular ComplexesHigh-resolution Cryo-EMParticle Box SizeCTF EstimationMotion CorrectionDose-weightedDose-unweightedParticle Picking

Read Article

Cite this Article

Moriya, T., Saur, M., Stabrin, M., Merino, F., Voicu, H., Huang, Z., Penczek, P. A., Raunser, S., Gatsogiannis, C. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J. Vis. Exp. (123), e55448, doi:10.3791/55448 (2017).

Download .ris file

Copy

Share Video

.

Copy