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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析

49,814 Views

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13:28 min

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May 16, 2017

DOI:

10.3791/55448-v

DOI:
10.3791/55448-v

•

13:28 min
May 16, 2017

•

12 Views
, , , , , , , ,
1Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, 2Biyokimya ve Moleküler Biyoloji Anabilim Dalı,The University of Texas Medical School at Houston

Özet

Automatically generated

本文介绍了使用软件套件SPHIRE处理低温EM图像的协议。目前的协议可以应用于几乎所有瞄准近原子分辨率的单粒子EM项目。

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Cite this Article

Moriya, T., Saur, M., Stabrin, M., Merino, F., Voicu, H., Huang, Z., Penczek, P. A., Raunser, S., Gatsogiannis, C. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J. Vis. Exp. (123), e55448, doi:10.3791/55448 (2017).

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