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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
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March 12, 2017
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We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.
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Dhoondia, Z., Tarockoff, R., Alhusini, N., Medler, S., Agarwal, N., Ansari, A. Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach. J. Vis. Exp. (121), e55446, doi:10.3791/55446 (2017).
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