/
/
Optimierte Einzelzelle TCR Isolation und Generierung von retroviralen Vektoren für In-Vitro und In Vivo Ausdruck menschlichen TCRs
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Optimierte Einzelzelle TCR Isolation und Generierung von retroviralen Vektoren für In-Vitro und In Vivo Ausdruck menschlichen TCRs
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Bölümler
- 00:00Başlık
- 01:42Single cell sort and reverse transcription reaction
- 03:37Multiplex-nested PCR
- 08:56Subcloning TCR alpha and beta chains
- 09:56Verification of TCR expression
- 10:46Conclusion
