This content is Free Access.
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
9,676 Views
•
11:09 min
•
April 17, 2018
DOI:
Özet
Automatically generated
Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.
Explore More Videos
HS-MRM AssayHost-cell ProteinsProtein BiopharmaceuticalsLiquid ChromatographyIon MobilityQTOF Mass SpectrometryPeptide QuantificationProtein CharacterizationQuantitative ProteomicsLow-abundant ProteinsPeptide SeparationGradient ElutionIon Mobility SeparationHigh-resolution Mass SpectrometryHigh-definition MSECollision EnergyLock Mass Correction
Read Article
Cite this Article
Doneanu, C., Fang, J., Alelyunas, Y., Yu, Y. Q., Wrona, M., Chen, W. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry. J. Vis. Exp. (134), e55325, doi:10.3791/55325 (2018).
Copy