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Gezielt<em> In Situ</em> Mutagenese von Histon-Gene in Bäckerhefe
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JoVE Journal Genetik
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
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Gezielt<em> In Situ</em> Mutagenese von Histon-Gene in Bäckerhefe

DOI:
10.3791/55263-v

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08:48 min

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January 26, 2017

•

,
1Biology Department,Hendrix College

Bölümler

  • 00:05Başlık
  • 00:46Generation and Purification of PCR Products Corresponding to Two Mutated and Partially Overlapping Histone Gene Fragments
  • 02:06Fusion PCR of the Two Partially Overlapping Fragments to Generate Full Size PCR Products for Integration
  • 03:20Co-transformation, Screen for 5-FOA-resistant Transformants, Purification of 5-FOA-resistant Colonies, and Loss of Backbone Plasmid
  • 05:11Results: In Situ Mutagenesis of HHT2 in Yeast
  • 07:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A strategy for generating mutations in histone genes at their endogenous location in Saccharomyces cerevisiae is presented.

Etiketler

Targeted In Situ MutagenesisHistone GenesBudding YeastPCRFusion PCRTemplate DNAAgarose GelURA3 Gene5-FOA PlateReplica Plating

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